{
  "metadata": {
    "name": "MR. DIXANT KARKI",
    "cr_no": "393286",
    "age_sex": "33Y/Male",
    "referred_doctor": "Dr. Vineet Talwar/Varun/Arpit",
    "sample_type": "Tumor Tissue (FFPE block) –B/9562/26 [2-2]",
    "diagnosis": "Rectal carcinoma",
    "tumor_fraction": "~50%",
    "order_no": "",
    "order_date": "04.08.2026",
    "receiving_date": "03.08.2026(FROM OPD)",
    "reporting_date": "24.08.2026",
    "lab_id": "2026238-2049"
  },
  "report_information": {
    "panel_title": "NGS Expanded Solid Tumor Panel"
  },
  "clinical_content": {
    "sections": [
      {
        "heading": "Report Highlights",
        "order": 1,
        "content": "No clinically significant alteration(s) detected in the genes analyzed.",
        "page": 1,
        "source_lines": [
          52
        ],
        "children": [
          {
            "heading": "Genomic Assembly",
            "order": 2,
            "content": "GrCH 37(hg19)",
            "page": 1,
            "source_lines": [
              53
            ]
          },
          {
            "heading": "Assay Information and Methodology",
            "order": 3,
            "content": "The assay utilizing a minimum of 10ng of DNA and 10ng of RNA at 500X coverage provides an analytical sensitivity of more than equal to 5\npercent for DNA-based genetic alteration.\nVariants of strong and potential clinical significance are only reported in somatic panels.",
            "page": 1,
            "source_lines": [
              55,
              92,
              93,
              96
            ],
            "children": [
              {
                "heading": "Test Description",
                "order": 4,
                "content": "– The Oncomine Precision Assay analyzes 78 variants, including mutations (45), CNVs (14), and fusion variants (19), across 50 key\ngenes. This has been run on the Genexus Integrated Sequencer.  The Genexus Integrated Sequencer automates NGS library preparation, sequencing,\nand analysis",
                "page": 1,
                "source_lines": [
                  58,
                  59,
                  60
                ]
              },
              {
                "heading": "Genes Analyzed for SNVs and indels",
                "order": 5,
                "content": "AKT1, AKT2, AKT3, ALK, AR, ARAF, BRAF, CDK4, CDKN2A, CHEK2, CTNNB1, EGFR, ERBB2, ERBB3, ERBB4, ESR1, FGFR1,\nFGFR2, FGFR3, FGFR4, FLT3, GNA11, GNAQ, GNAS, HRAS, IDH1, IDH2, KIT, KRAS, MAP2K1, MAP2K2, MET, MTOR, NRAS, NTRK1, NTRK2, NTRK3, PDGFRA,\nPIK3CA, PTEN, RAF1, RET, ROS1, SMO, TP53",
                "page": 1,
                "source_lines": [
                  63,
                  64,
                  65
                ]
              },
              {
                "heading": "Genes Analyzed for Rearrangements",
                "order": 6,
                "content": "ALK, BRAF, ESR1, FGFR1, FGFR2, FGFR3, MET, NRG1, NTRK1, NTRK2, NTRK3, NUTM1, RET, ROS1, RSPO2, RSPO3.\nIntra-genetic fusions in EGFR, MET AND AR",
                "page": 1,
                "source_lines": [
                  68,
                  69
                ]
              },
              {
                "heading": "Genes Analyzed for Copy Number Variations",
                "order": 7,
                "content": "ALK, AR, CD274, CDKN2A, EGFR, ERBB2, ERBB3, FGFR1, FGFR2, FGFR3, KRAS, MET, PIK3CA, PTEN",
                "page": 1,
                "source_lines": [
                  72
                ]
              },
              {
                "heading": "Quality Metrics",
                "order": 8,
                "metrics": {
                  "DNA": {
                    "status": "PASSED",
                    "Mean depth of coverage": "5701x",
                    "Uniformity": "96.40%"
                  },
                  "RNA": {
                    "status": "PASSED",
                    "Total mapped fusion reads": "183,310 (Cutoff>20,000)"
                  }
                },
                "page": 1,
                "source_lines": [
                  75,
                  78,
                  81,
                  83,
                  86,
                  89
                ]
              },
              {
                "heading": "Disclaimer",
                "order": 9,
                "content": "The information in this report does not constitute a treatment recommendation or recommendation to not use any specific therapeutic agent, and\nit should not be interpreted as treatment advice. Decisions concerning patient care and treatment rest solely within the discretion of the patient's treating\nphysician.",
                "page": 1,
                "source_lines": [
                  98,
                  99,
                  100
                ]
              }
            ]
          }
        ]
      }
    ]
  },
  "authorization": {
    "performed_by": "Mamta Arya Scientist C\nAmit Negi Scientist A\nMolecular Diagnostics",
    "reviewed_by": "Dr Rushali Saxena\nAttending Consultant, Pathology",
    "approved_by": "Dr. Anurag Mehta\nPrincipal Director Laboratory Services\nRajiv Gandhi Cancer Institute & Research Centre.\nRohini, New Delhi."
  },
  "processing": {
    "source_file": "/app/uploads/29380_NGS Expanded Panel for Solid Tumor Tissue.pdf",
    "content_hash": "58e25acb4698ec2c9c5c9f296c02844b4e46d20e488adee1ddc5f3cee6ca9434",
    "processed_at": "2026-09-12T10:03:49.423693+00:00",
    "pipeline_version": "1.0.0",
    "pipeline_name": "molecular",
    "pages": 1,
    "sections_detected": 1,
    "warnings_count": 0,
    "skipped_image_blocks": 1,
    "skipped_figure_lines": 0,
    "content_coverage": 0.9713,
    "validation_status": "PASSED",
    "validation_failures": [],
    "validation_warnings": [],
    "header_layout": "B"
  }
}